摘 要：目的：通过观察离心运动及针刺干预对Wnt/βcatenin信号通路主要信号分子的影响，探讨该通路在骨骼肌损伤修复中的作用。方法：将雄性SD大鼠随机分为安静组（C）、安静针刺组（CA）、运动组（E）与运动针刺组（EA），E与EA组进行坡度为-16°的一次性跑台运动；取大鼠股中间肌，实时定量PCR检测Wnt1、Wnt3a mRNA表达，免疫印迹法检测βcatenin、GSK-3β蛋白磷酸化水平。结果：1）CA组Wnt1 mRNA表达均低于C组，且Wnt3a mRNA在12 h、72 h及120 h显著高于C组（P<0.05）； CA组72 h GSK-3β活性与120 h βcatenin磷酸化水平显著低于C组（P<0.05）。2）EA组Wnt1、Wnt3a mRNA表达的升高程度低于E组。72 h E组和EA组GSK-3β活性与βcatenin磷酸化水平均显著低于C组（P<0.05），且E组βcatenin磷酸化水平显著低于EA组（P<0.05）。 结论：一次性离心运动及运动后针刺能上调Wnt1、Wnt3a mRNA表达，降低GSK-3β活性与βcatenin磷酸化水平，针刺有上调βcatenin磷酸化水平的作用。
关键词：离心运动；针刺；Wnt/βcatenin信号通路；卫星细胞
中图分类号：G804.23 文献标识码：A 文章编号：1007-3612（2012）10-0056-07
Effects of Eccentric Exercise and Acupuncture on the Wnt/βcatenin Signaling in Rat Skeletal Muscle
SHI Jipeng1， XU Hongqi1， ZHANG Xuelin2， GAO Xiaojuan3， ZHOU Yue4， LI Junping4， LUO Lina4， WANG Ruiyuan4
（1. Northeast Normal University， Changchun 130024， Jilin China；2. Shijiazhuang College， Shijiazhuang 050035， Hebei China； 3.Zhengzhou University， Zhengzhou 450001， Henan China； 4. Beijing Sport University， Beijing 100084， China）
Abstract：Objective：To observe the effects of eccentric exercise and acupuncture on Wnt/βcatenin signaling， then analyze its effects on skeletal muscle damage repairment. Methods：Male SD rats were divided into four groups：E and EA group were submitted to a single bout of eccentric exercise on a treadmill down a 16°decline. Vastus intermedius muscle was obtained in 12h， 24h， 48h， 72h， 120h postexercise and/or after needling. Wnt1 and Wnt3a mRNA expression were detected by RTPCR，phosphorylation of βcatenin and GSK3β were detected by western blot. Results：1） Wnt1 mRNA expression of CA group were lower than C group， Wnt3a mRNA expression of CA group were significantly higher than C group in 12h、72h and 120h （P<0.05）. GSK3β activity of CA group decreased significantly compared with C group in 72h （P<0.05）， phosphorylation of βcatenin also decreased significantly compared with C group in 120h （P<0.05）. 2） Wnt1 and Wnt3a mRNA expression of EA group were lower than E group. GSK3β activity and phosphorylation of βcatenin of E and EA group decreased significantly compared with C group in 72h （P<0.05）， and phosphorylation of βcatenin in E group decreased significantly than EA group （P<0.05）. Conclusion： A single bout of eccentric exercise and needling after exercise can upregulate the mRNA expression of Wnt1 and Wnt3a， decrease the activity of GSK3β and phosphorylation of βcatenin， needling has the effect on upregulating phosphorylation of βcatenin.